Bacillus subtilis (natto) Plasmid Responsible for Polyglutamate Production Encoding r-Glutamyltranspeptidase
نویسندگان
چکیده
منابع مشابه
Elimination of plasmid-linked polyglutamate production by Bacillus subtilis (natto) with acridine orange.
Treatment of Bacillus subtilis (natto) strains Asahikawa, F, and M with acridine orange resulted in the conversion of approximately 64.2% of the Asahikawa population, 22.4% of the F population, and 9.2% of the M population to polyglutamate-nonproducing colonies. Such curing is suggestive of the involvement of plasmid DNA. Samples of cleared lysates of both parental and their cured strains were ...
متن کاملCharacterization of the replication region of plasmid pLS32 from the Natto strain of Bacillus subtilis.
Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN gene starts 167 nucleotides upstream from the translational start site of rep...
متن کاملA polyglutamate form of citrovorum factor synthesized by Bacillus subtilis.
The discovery of the citrovorum factor (CF) in yeast and in liver (Sauberlich and Baumann, 1948) was accompanied by the observation that a portion of the material existed in a bound form, from which it was liberated by incubation with an enzyme system ("conjugase") found in hog kidney (Bird et al., 1946). Bound forms of CF were found by Iwai et al. (1954) in higher plants, and these also contai...
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An industrial strain of Bacillus subtilis (natto) was used to produce poly-gamma-DL-glutamate (gammaPGA), a polymer of DL-glutamate linked by a gamma-peptide bond. In spite of efforts to improve gammaPGA production by modifying the medium, little attention has been paid to the expression of the gammaPGA synthetase gene. In this study, we investigated the expression of the gammaPGA synthetic gen...
متن کاملConstruction of physical maps of Bacillus subtilis (natto) strains.
The putative physical map of a Bacillus subtilis (natto) strain has been constructed to demonstrate the difference from the strain B. subtilis Marburg. B. subtilis (natto) strains are closely related to the Marburg 168trpC2 strain whose genome was sequenced. Their genome size and organization are also assumed to be similar. The putative SfiI and I-CeuI physical map of a natto strain BEST195 rev...
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ژورنال
عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University
سال: 1985
ISSN: 0023-6152
DOI: 10.5109/23815